伊人是香蕉大人国产达人,国产激情视频在线观看大全,av天堂新资源在线

Info

Title:: Study on the Repair of Articular Cartilage Defects by EDC/NHS Cross-linked Recombinant Collagen Hydrogel

Author(s):: HUANG Changjin; LEI Huan; TANG Xiaojun; 1. Hospital of Plastic and Reconstructive Surgery, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100144, China 2. College of Chemical Engineering, Northwestern University, Xi’an 710069,China 3. Institute of Biomedical Research, Northwestern University, Xi’an 710069, China

Keywords:: EDC/NHS; cross-linking; recombinant collagen; injectable hydrogel; articular cartilage repair

CLC:

PACS:: R684;R318.08

DOI:: 10.7502/j.issn.1674-3962.202401011

DocumentCode:

Abstract:: Articular cartilage injuries and defects are a common and severe clinical challenge without effective treatment, and collagen-based hydrogels is a hotspot for cartilage tissue engineering, but there are still great challenges in developing collagen products with good biocompatibility and safe crosslinking, and the effectiveness of cartilage repair needs improving. In this study, recombinant type I collagen and recombinant type III collagen were crosslinked by EDC/NHS to prepare injectable hydrogels. The two hydrogels exhibited good three-dimensional pore structures, and excellent mechanical properties as well as degradability. It was found that recombinant type I collagen hydrogel (Gel-Ⅰ) and recombinant type III collagen hydrogel (GelⅢ) could promote the glycosaminoglycans secretion of HBMSCs. The effects of the hydrogels on the chondrogenic differentiation of BMSC were analyzed by RFqPCR, and it was found that Gel-Ⅰ significantly up-regulated COLII gene and SOX9 gene, and Gel-Ⅲ significantly up-regulated SOX9 gene. In the rabbit knee articular cartilage repair model, compared with the blank and Gel-Ⅲ groups, Gel-Ⅰ group showed the significant advantage in bone volume size, subchondral bone had been formed and effectively restored, the number and thickness of trabeculae were the highest, the trabeculaes were more uniformly distributed, and the thickness of the new cartilage at the defect was the same as that of the neighbouring cartilage. In conclusion, recombinant type I collagen hydrogel has great potential for clinical application in terms of the effectiveness and safety of cartilage repair.

亚洲国产aⅴ成人精品无吗,中文字幕999精品,人妻人人妻a乱aa乱,日韩三级aaaaa,亚洲午夜成人精品无码色欲,国产第一页第二页av,老熟女北岛玲888av,99re热这里只有精品18,久久精品中文字幕久久

Study on the Repair of Articular Cartilage Defects by EDC/NHS Cross-linked Recombinant Collagen Hydrogel(PDF)

MATERIALS CHINA[ISSN:1674-3962/CN:61-1473/TG]

Info

References

Memo

Common functions

Navigate

Tools

Statistics