文章信息/Info

Title:

Progress in Optimization of Mesenchymal Stem Cells by Three-Dimensional Spheroid Culture

文章編號:

1674-3962(2020)04-0278-09

作者:

梁瑜1; 2; 喬勇2; 劉星志2; 3; 徐中娟2; 3; 朱小立1; 索廣力2

（1.上海大學(xué)生命科學(xué)學(xué)院，上海 200444）（2.中國科學(xué)院蘇州納米技術(shù)與納米仿生研究所，江蘇 蘇州 215123）（3.中國科學(xué)技術(shù)大學(xué)納米技術(shù)與納米仿生學(xué)院，安徽 合肥 230026）

Author(s):

LIANG Yu1; 2;  QIAO Yong2;  LIU Xingzhi2; 3;  XU Zhongjuan2; 3;  ZHU Xiaoli1;  SUO Guangli2

（1.School of Life Sciences, Shanghai University, Shanghai 200444, China) （2.Suzhou Institute of Nano�睺ech and Nano�睟ionics, Chinese Academy of Sciences, Suzhou 215123, China) （3. School of Nano�睺ech and Nano�睟ionics, University of Science and Technology of China, Hefei 230026, China）

關(guān)鍵詞:

三維培養(yǎng); 間充質(zhì)干細(xì)胞; 干細(xì)胞優(yōu)化; 臨床應(yīng)用; 細(xì)胞球

Keywords:

three-dimensional culture (3D culture);  mesenchymal stem cells (MSCs);  stem cell optimization;  clinical application;  spheroid

分類號:

R318

DOI:

10.7502/j.issn.1674-3962.201912014

文獻(xiàn)標(biāo)志碼:

A

摘要:

間充質(zhì)干細(xì)胞（mesenchymal stem cells, MSCs）源于發(fā)育早期的中胚層，因其來源廣泛、具有多向分化潛能、低免疫原性和自我更新能力，在組織工程和再生醫(yī)學(xué)應(yīng)用中顯示出巨大的潛力，也是當(dāng)前基礎(chǔ)研究和臨床研究中應(yīng)用最多的一類干細(xì)胞。然而，間充質(zhì)干細(xì)胞的臨床應(yīng)用面臨許多挑戰(zhàn)，比如治療所需細(xì)胞數(shù)量巨大，細(xì)胞質(zhì)量存在異質(zhì)性，細(xì)胞體內(nèi)移植后存活率低，以及二維（two-dimensional, 2D）貼壁培養(yǎng)導(dǎo)致間充質(zhì)干細(xì)胞特征衰減等。三維（three-dimensional, 3D）成球培養(yǎng)可以更好地模擬體內(nèi)微環(huán)境，且大量的研究證明，3D成球培養(yǎng)增強(qiáng)了間充質(zhì)干細(xì)胞的細(xì)胞存活和因子分泌能力，促進(jìn)了干細(xì)胞特征維持、細(xì)胞遷移和血管生成，在臨床醫(yī)學(xué)領(lǐng)域具有廣闊的應(yīng)用前景。基于此，綜述了體外3D成球培養(yǎng)的方法、3D成球培養(yǎng)優(yōu)化的間充質(zhì)干細(xì)胞的生物學(xué)特性及應(yīng)用，并對3D成球培養(yǎng)未來的研究方向進(jìn)行展望。

Abstract:

As an important branch of stem cell, mesenchymal stem cells (MSCs) are derived from the mesoderm at early embryonic development, which have shown great potentials in tissue engineering and regenerative medicine applications due to their extensive sources, multilineage differentiation potential, low immunogenicity and self�瞨enewal ability. However, MSCs are still confronting challenges in clinical applications due to requirement of large quantity of cells for therapy, heterogeneity of cell quality, low survival ability after transplantation, the attenuation of MSCs characteristics in two�瞕imensional (2D) culture and ambiguous mechanism of MSCs function for disease therapy. Three�瞕imensional (3D) spheroid culture is widely regarded as a more preferable and closer physiological microenvironment for cells growth. Studies reported that 3D spheroid culture enhanced the characteristics of MSCs on cell survival, factor secretion, stemness maintenance, migration, anti�瞫enescence in�瞯itro and enhanced the capacities of anti�瞚nflammatory, angiogenesis, tissue repair and regeneration in�瞯ivo. Therefore, 3D culture has broad application prospects in clinical application. In view of this, this article summarized the methods of 3D spheroid culture in vitro, and the spheroid biological characteristics and application briefly. Meanwhile, the prospects in future also have been discussed.