文章信息/Info

Title:

Preparation of Orthosiphon Stamineus Nano-Dispersion and Its Effect on Uric Acid

文章編號:

1674-3962(2021)04-0297-04

作者:

劉睿1; 羅超2; 朱君1; 2

（1. 納米技術及應用國家工程研究中心，上海 200241）（2. 上海健康醫(yī)學院 上海市分子影像學重點實驗室，上海 201318）

Author(s):

LIU Rui1; LUO Chao2; ZHU Jun1; 2

（1. National Engineering Research Center for Nanotechnology，Shanghai 200241，China）（2. Shanghai Key Laboratory of Molecular Imaging，Shanghai University of Medicine & Health Sciences， Shanghai 201318，China）

關鍵詞:

貓須草; 納米技術; 尿酸; 肝副作用; 細胞毒性

Keywords:

orthosiphon stamineus; nanotechnology; uric acid; liver side effect; cell cytotoxicity

分類號:

TB383

DOI:

10.7502/j.issn.1674-3962.202101004

文獻標志碼:

A

摘要:

納米中藥是近年來迅速發(fā)展起來的前沿科技領域。納米技術和傳統(tǒng)中藥制劑的有機結合，有力地推動了醫(yī)藥科技的發(fā)展。以貓須草為研究對象，采用剪切、研磨和高壓均質(zhì)等方法制備了貓須草納米分散液，獲得了平均粒徑約為100 nm的均勻、穩(wěn)定的分散體系，研究了納米分散液降尿酸（UA）的作用。結果表明：相對于高尿酸模型組，納米分散液對UA的抑制率為22.1%；相對于水提液實驗組，納米分散液表現(xiàn)出更好的降尿酸作用。通過進一步考察其對肝臟指標的影響，探究了納米分散液的毒副作用。相對于高尿酸模型組，納米分散液對肝臟谷丙轉氨酶（ALT）和谷草轉氨酶（AST）的表達水平未見明顯影響，這表明納米分散液未引起明顯的肝損傷。最后，通過細胞實驗證明了納米分散液具有較低的細胞毒性。該實驗表明貓須草納米分散液的劑型在降尿酸方面具有潛在的應用價值，為納米中草藥的應用提供了理論依據(jù)。

Abstract:

Nano-traditional Chinese medicine is a new research field that is developing rapidly in recent years. The combining of nanotechnology with traditional Chinese medicine produces an enormous influence on the development of medical science. In this paper, the preparation method of orthosiphon stamineus nano-dispersion is developed by shear grinding and high pressure homogenization. A uniform and stable nano-dispersion with average particle size of about 100 nm is obtained. Furthermore, the effect of the nano-dispersion on reducing uric acid (UA) is investigated. Compared with the model group, the inhibition rate of the nano-dispersion on UA is 22.1%, and compared with the water extract experimental group, the nano-dispersion shows a better effect on reducing UA. Additionally, the side effects of the nano-dispersion are evaluated by investigating the liver indexes, which shows that nano-dispersion causes less liver injury through detecting alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of liver compared to that of the model group. Finally, the cytotoxicity of nano-dispersion is proved by cell experiment. The results show that the orthosiphon stamineus nano-dispersion has potential application in reducing UA, which can provide a theoretical basis for the reasearch of nano-dispersion about traditional Chinese medicine.